One or more volunteers were required to donate their DNA for Unique ethical and political requirements with respect to the source DNA for library construction, because never before hasĪn individual's genetic blueprint been deciphered completely. Was completed on June 26, 2000, and a high-quality version will become accessible by 2003. The initial draft version of the human DNA sequence Large centers in the United States and the United Kingdom (the G5 group) along with three smaller centers in France, Germany,Īnd Japan (the G8 group) are the major contributors to the sequencing effort. The main goal of the publicly funded human genome project is to completely determine the human genomic DNA sequence. The sequence data described in this paper have been submitted to the GenBank data library under accession nos. Sequencing, clone fingerprinting, high-throughput sequence analysis and as a source of mapped clones for diagnostic and functional This BAC library, designated as “RPCI-11,” has been used widely as the central resource for insert-end Eighteen BACs (11%) revealed minor insert rearrangements,Īnd none was chimeric. The quality of the library was assessedīy extensive analysis of 169 clones for rearrangements and artifacts. Were generated by partial digestion with EcoRI (library segments 1–4: 24-fold) and MboI (segment 5: sixfold) and cloned into the pBACe3.6 and pTARBAC1 vectors, respectively. The DNA was obtained fromĪ single anonymous volunteer, whose identity was protected through a double-blind donor selection protocol. A 30-fold redundant human bacterial artificial chromosome (BAC) library with a large average insert size (178 kb) has beenĬonstructed to provide the intermediate substrate for the international genome sequencing effort.
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